Analytical Session
Qun Song, Jiangnan University,Wuxi,Jiangsu,China
Co-author(s): Jinjing Wang and Qi Li, Jiangnan University, Wuxi, China
ABSTRACT: A simple and sensitive method had been developed
for the determination of proteinase A activity using a resonance light
scattering (RLS) technique. The method was based on the principle that
TCA and trace protein can form association particles and the particles’
concentration proportioned resonance scattering light intensity under
certain conditions. In this study, several methods were compared for
proteinase A activity measurement. In the meantime, the influence of
temperature, pH value, and TCA concentration were investigated. This new
method was fast and convenient and had a low detection limit (0.595
g/mL) and wide linear range (1–200 g/mL). The system had better
tolerance against interfering substances, and the reaction system
remained stable for at least 150 min. The method was satisfactorily
applied to the determination of trace proteinase A activity.
Qun
Song, a master’s degree student in biotechnology at Jiangnan University
in China, is mainly engaged in research on detection methods and
secretion of proteinase A.
VIEW PRESENTATION 106
