MATTHEW ECKERT (1)
(1) Covance Laboratories, Inc., Madison, WI
The listing of 4-methylimidazole (4-MeI) on California’s Proposition
65 list has raised interest in the need to analyze 4-MeI accurately and
efficiently. Several methods for the analysis of 4-MeI are commonly
employed, including LC using UV or mass spectrometric detection and
GC-MS after ion-pair extraction and derivatization. This presentation
focuses on analysis of 4-MeI using LC with mass spectrometric detection,
based on methodology previously developed by Klejdus et al. (J.
Separation Sci., 29:378-384, 2006). With the current availability of an
inexpensive stable, labeled internal standard, the methodology above can
be further optimized. In addition, certain matrices can be analyzed by
direct injection after dilution, depending on the LOD desired. Finally,
the application of the above methodology to simultaneous analysis of
2-MeI and 4-MeI is also addressed.
Matthew Eckert joined Covance Laboratories in 1999. Over the last 12
years he has held positions as a toxicology dose formulation technician
and as a chemist, both in bioanalytical chemistry and nutritional
chemistry/food safety.
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