E. HUMSTON-FULMER (1), J. Binkley (1); (1) LECO Corporation, Saint Joseph, MI, U.S.A.

Poster

Characterization of flavor and aroma compounds provides useful information for the food and beverage industries that can provide insight into quality control, process optimization, and sensory characteristics. Non-targeted methods that isolate and identify individual analyte components within the complex food matrix can provide a great deal of information. Here, methods were developed that utilize gas chromatography (GC) to separate individual analyte components from each other and time-of-flight mass spectrometry (TOFMS) for identification of the individual analytes. Aroma profile samples were prepared for analysis with headspace solid-phase microextraction (HS-SPME) to collect and concentrate the volatile and semi-volatile aroma and flavor compounds associated with a sample. These techniques were utilized to investigate both processing of raw materials and characterization of finished products. Changes in the aroma profile associated with the duration of boil time of hops were determined. The differences were readily apparent in the overall sample complexity and also clearly noted by changes to individual analyte concentrations. Characterization and comparison of final products was also accomplished with these analytical techniques. Principal component analysis (PCA) was used to investigate the similarities and differences of the sample groups, with clear differences determined. These methods allowed for comparing sample types by their overall chromatographic features and by individual analyte differences in order to differentiate changes to a production process and to distinguish beer samples.

Elizabeth Humston-Fulmer is an Applications Chemist at LECO Corporation.