VIEW ARTICLE    DOI: 10.1094/ASBCJ-53-0078

Determination of Deoxynivalenol in Barley, Malt, and Beer by Gas Chromatography-Mass Spectrometry. David J. Hastings and Lauren E. Stenroos, The Stroh Brewery Company, Detroit, MI. J. Am. Soc. Brew. Chem. 53(2):78-81, 1995. Accepted October 18, 1994.

A number of analytical methods are currently available for the determination of deoxynivalenol in barley, malt, and beer. They usually involve lengthy, time-consuming cleanup regimes. A method developed for barley and malt employs the Romer 225 mycotoxin cleanup column, which yields clean, low-volume extracts that can be quickly derivatized and analyzed by gas chromatography-mass spectrometry. Extraction recoveries were typically >90% with correlation coefficients of >0.999. In a method developed for beer, extraction with ethyl acetate, without cleanup, yielded correlation coefficients of >0.999 and an extraction efficiency of 70%. Keywords: Beer, Deoxynivalenol, Gas chromatography, Malt, Vomitoxin