VIEW ARTICLE    doi:10.1094/ASBCJ-2010-1119-01

Isolation and Characterization of Bacteriophages That Inhibit Strains of Pediococcus damnosus, Lactobacillus brevis, and Lactobacillus paraplantarum That Cause Beer Spoilage. David Kelly, Department of Biological Sciences, Cork Institute of Technology, Bishopstown, Cork, Ireland; Horst Neve, Max Rubner-Institute, Federal Research Institute of Nutrition and Food, Department of Microbiology and Biotechnology, Kiel, Germany; Olivia McAuliffe and R. Paul Ross, Biotechnology Department, Teagasc, Moorepark Food Research Centre, Fermoy, Cork, Ireland; Elke K. Arendt, Department of Food and Nutritional Sciences, University College Cork, Ireland; and Aidan Coffey (1), Department of Biological Sciences, Cork Institute of Technology, Bishopstown, Cork, Ireland. (1) Corresponding author. Department of Biological Sciences, Cork Institute of Technology, Bishopstown, Cork, Ireland. E-mail: <aidan.coffey@cit.ie>; Phone: 353-21-4326834; Fax: 353-21-4326308. J. Am. Soc. Brew. Chem. 69(1):8-12, 2011.

The aim of this study was to isolate and characterize bacteriophages against Pediococcus and Lactobacillus strains that cause spoilage in brewing processes. A number of beer-spoilage bacteria were isolated from breweries and characterized by 16S rRNA typing. Five distinct P. damnosus phages and four Lactobacillus phages, which lysed both L. brevis and L. paraplantarum, were isolated from municipal sewage and farmyard slurries. A rapid DNA isolation method was used to isolate DNA of sufficient purity for restriction endonuclease digestion from de Man, Rogosa, and Sharpe (MRS) broth. Phages were analyzed using restriction digest and transmission electron microscopy and shown to be in the family Siphoviridae, with genomes ranging in size from 40 to 50 kb. All phages were characterized and shown to be distinct. This study identifies five novel phages against P. damnosus. Phages for this species are very rare in the scientific literature. Four novel phages against L. brevis and L. paraplantarum are also identified. These phages may have an application in the biocontrol of beer-spoilage bacteria. Keywords: Bacteriophage, Beer, Biocontrol, Lactobacillus, Pediococcus, Phage DNA isolation

El objetivo de este estudio fue aislar y caracterizar los bacteriófagos contra cepas de Lactobacillus y Pediococcus que causan deterioro en los procesos de elaboración de la cerveza. Un número de bacterias de deterioro de cerveza fueron aisladas de fábricas de cerveza y se caracteriza por ARNr 16S. Cinco distintos fagos de P. damnosus y cuatro fagos de Lactobacillus, que tanto lisis L. brevis y L. paraplantarum, fueron aislados de las aguas residuales municipales y lodos de corral. Un método rápido aislamiento del ADN se utilizó para aislar el ADN de suficiente pureza para la digestión con endonucleasas de restricción del caldo de Man, Rogosa, y Sharpe (MRS). Los fagos fueron analizados mediante el resumen de la restricción y la microscopía electrónica de transmisión y se demostró que eran en la familia Siphoviridae, con genomas que varían en tamaño desde 40 hasta 50 kb. Todos los fagos se caracterizaron y se demostró que ser distintos. Este estudio identifica cinco fagos novela contra P. damnosus. Los fagos a esta especie son muy raros en la literatura científica. Cuatro nuevos fagos contra L. brevis y L. paraplantarum también se identifican. Estos fagos pueden tener una aplicación en el control biológico de bacterias de deterioro de cerveza. Palabras claves: Bacteriófago, Bacteriófago aislamiento del ADN, Cerveza, Control biológico, Lactobacillus, Pediococcus